Composite
Part:BBa_K4724024:Design
Designed by: Qingqing Zheng Group: iGEM23_NJTech-China-A (2023-10-10)
IsPETase-Linker- LSChi4CBM -6xHisTag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 790
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal NgoMIV site found at 969
Illegal AgeI site found at 546 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use Linker to fuse the domain to the IsPETase protein and connect 6xHis Tags behind the domain for easy purification.
Source
Designed by NJTech-China-A.